An Integrated PCR Colony Hybridization Approach to Screen cDNA Libraries for Full-Length Coding Sequences

An Integrated PCR Colony Hybridization Approach to Screen cDNA Libraries for Full-Length Coding Sequences

BACKGROUND cDNA-Amplified Fragment Length Polymorphism (cDNA-AFLP) is a commonly used technique for genome-wide expression analysis that does not require prior sequence knowledge. Typically, quantitative expression data and sequence information are obtained for a large number of differentially expressed gene tags. However, most of the gene tags do not correspond to full-length (FL) coding seque...

An integrated analysis and database system for full-length cDNA.

Annotation and database system of full-length cDNA sequences was developed. As the components of the system, ORF annotation system, functional annotation system based on database search results, mapping annotation system, and integrated retrieval and display system were developed. In the ORF annotation system integrated analyses using conventional tools are performed and useful retrieval interf...

PCR-based method for isolation of full-length clones and splice variants from cDNA libraries.

Most cDNA library screening procedures do not distinguish between full-length and incomplete clones and therefore may yield incomplete cDNA fragments. Thus, there is a widespread need for a method allowing the efficient selection of full-length clones. I present a rapid, PCR-based method that allows the simultaneous screening of > 10(6) cDNAs. The longest cDNA is identified in the first step so...

An Integrated Database System for Full-Length Human cDNA in NEDO Full-Length Human cDNA Sequencing Project

Assessment of Redundancy and Full-Length Rate of Full-Length Enriched cDNA Libraries

Collection of full-length genes requires libraries with full-length cDNA insert, large-scale sequencing, library assessment, and high-speed sequence clustering. Here we focus on computational methods, such as newly developed computer programs, since our experimental methods had been published previously. Our purpose is the collection of full-length cDNAs, therefore the proportion of full-length...